Strong, Review of the Golgi Method. 127 



"The modification which I have adopted with my subh- 

 mate method is as follows: 



" The pieces which have been proven to be successfully- 

 impregnated are imbedded in celloidin in the usual way and 

 cut with the microtome. The sections are then subjected to 

 the following treatment : 



" (i) Washing in distilled water. 



•' (2) Immersion one or two minutes (they can also re- 

 main several minutes without injury) in the above fixing and 

 staining fluid. Several cubic centimeters of the fluid suffice 

 for many sections. The blackening can be observed with the 

 naked eye. 



*' (3) Careful washing in distilled water. 



" (4) If desired, light carmine stain to bring out the cell 

 bodies and nuclei in the fine interstitial nervous network. Acid 

 carmine is best adapted for this according to my experience and 

 I find especially suitable a dilution of this staining fluid with 

 acetic acid and alcohol (equal parts). The fluid into which the 

 sections are brought must have a deep red color. 



"(5) Repeated washing in water and then successive 

 transference into alcohol and clove oil and finally mounting in 

 Canada balsam or damar in the usual way. 



" Preparations treated in this way possess, besides the 

 above mentioned advantages, the additional one that the fine 

 powdery precipitate does not after a while appear. This pre- 

 cipitate almost always, if there have not been previous repeated 

 and long continued washings, at last spoils the preparations 

 prepared according to the original method." 

 [to be continued.] 



Alum, 20 g. 



Ammonium sulphocyanicle, lo g. 



Sodium chloride, 40 g. 

 This mixture stands quiet for 8 days and is then filtered. 

 (b) Water, 100 g. 



Gold chloride, I g. 

 To prepare the bath one mixes 



of solution (a), 60 ccra. 



" " (b), 7 ccm. 



old, combined bath, 40 ccm. 

 For economy and convenience I use the fluid which has also served for ton- 

 ing, thus for this purpose almost useless. 



