HYPOPHYSIS AFTER CASTRATION 453 



In addition, there may be an actual lessening of the number of 

 stainable granules in some of the cells. Along the blood vessels, 

 the acidophiles usually still occupy more space than the baso- 

 philes, although in some localized areas they may be pressed 

 away by the enlarging basophiles. A definite example of this 

 was seen in sections of this age, in an area near the cephalic end, 

 where the basophiles occupied the greatest proportion of the 

 space, but the acidophiles were still there, although rather 

 flattened by the pressure of the neighboring cells. Another 

 exception to the usual cell type mixture, was seen in a limited 

 area towards the caudal end, where there were continuous rows 

 of acidophiles along the blood vessels, but no basophiles. This 

 arrangement of cells was seen at other ages also, in this area. 

 The reserve cell nuclei occur in small groups. These nuclei 

 show a uniform distribution of chromatin, with usually a nucleo- 

 lus. The cytoplasm is pale staining, finely granular and has 

 indistinct boundaries. 



Measurements of the large vacuoles in the basophiles show 

 them to average 12.6 x 9.2 yu, as contrasted with 10.8 x7.4iu at 

 two months after castration. The large cells containing these 

 vacuoles measure 21.5 x 15.4 m, with nuclei 7.7 x 5.9 ix. The 

 smaller basophiles without vacuoles measure 15.7 x 12.5 /x, with 

 nuclei 6.7 x 5.8 m- The normal cells at the same age in the con- 

 trol animals measure 14.5 x 11.0 m, with nuclei 6.7 x 5.6 ju, which 

 is but slightly less than the small sized basophiles of the experi- 

 mented hypophyses. 



Measurements of the acidophiles do not show any great 

 difference between the preceding age and this one. On the 

 average, the experimented acidophiles measure less than the 

 normal, but occasionally one finds a cell in the experimented 

 hypophysis quite as large as the largest normal acidophile. One 

 gets the impression that the stained acidophile granules are 

 becoming fewer in many cells of the experimented hypophysis, 

 and that these granules are not so closely arranged as in the 

 normal. 



