454 WILLIAM H. F. ADDISON 



Five months after castration {179 days old) 



The vacuoles in the basophiles are still larger than at the three 

 months after castration, and there appear to be more cells with 

 vacuoles than before. However, there are still some of about 

 the normal size and appearance, 'vvithout vacuoles, which rep- 

 resent cells of younger growth, but doubtless destined to become 

 vacuolated. There is a third variety of a basophilic type, per- 

 haps intermediate between the two preceding varieties, which 

 is nearly as large as many of the vacuolated cells, but is coarsely 

 granular, or perhaps better described as filled ■v^'ith numerous 

 very minute vacuoles. A definite group of these was seen near 

 the cephalic end of the section, and individual ones scattered 

 elsewhere. Along the blood vessels are still both basophiles 

 and acidophiles, the former becoming progressively larger and 

 more conspicuous, the latter becoming less conspicuous. The 

 acidophiles really seem fewer, but this may be due in part to 

 their separation by the large basophiles and also to being com- 

 pressed by them. Sometimes when situated between several of 

 these large cells they may be pressed into a triangular outline 

 At other places again, where they are seen to stain distinctly 

 and are not compressed, they appear of about the same size as in 

 the control. The vacuoles in the large basophiles measure 

 18.2 X 14.3 IX as contrasted with 12.6 x 9.2 /x at three months after 

 operation. These cells measure 20.3 x 18 m as contrasted with 

 15. 5x 11.3/xin the cells of the normal control animal. Measure- 

 ment of small non-vacuolated basophiles show them to be 13.2 x 

 6.7 ^J., and these must be cells still growing. The acidophiles 

 in the experimented animal measure 10.4 x 7.4 ^ and in the 

 normal animal 11.4 x 8.4 m, and these measurements are not 

 greatly different from those at the preceding stages. 



Seven months after castration {246 days old) 



The general appearance of the ventral glandular portion of the 

 experimented hypophysis at this period (fig. 5) is dominated 

 by the large number of vacuolated basophiles, distended with 

 their colloid-like secretion. The presence of these large cells 

 with the groups of smaller cells between gives it an appearance 



