456 WILLIAM H. F. ADDISON 



peared that some acidophiles lose their specifically staining 

 granules and in consequence have the appearance of reserve 

 cells. So that in the later stages after castration, the reserve 

 cells may contain among their number many cells which are 

 dedifferentiated acidophiles. 



Fichera ('05), who was the first to study castration changes 

 in the hypophysis, found increased size and weight of the entire 

 organ, and histologically an increase in number and size of 

 eosinophiles (acidophiles). The animals he used in his male' 

 series were domestic cattle, European buffaloes and domestic 

 fowl. Wittek ('13) who worked with a large series of individuals 

 of domestic cattle, agreed in finding that castration increases the 

 weight of the hypophysis. Histologically, however, he differed. 

 He described this part of the hypophysis as composed chiefly 

 of two cell types — eosinophiles (acidophiles) and chief cells 

 (reserve cells). True basophiles were only exceptionally seen. 

 After castration he found no change in cell types and no increase 

 of eosinophiles. 



Cimorini ('08) in rabbits and dogs found increase in volume 

 after castration, and an increase in number and size of the eosino- 

 phile cells. 



Kolde ('12), made observations principally upon the female 

 hypophysis, but as a minor part of his work also examined three 

 normal male rabbits and one castrated rabbit. He does not 

 indicate any different results in the two sexes, and gives his 

 results as an increase of eosinophiles, and the appearance of 

 special eosinophile cells, the protoplasm of which does not show 

 the usual even stain but is filled with little red granules, perhaps 

 colloid formation. 



Marrassini ('10) and Marrassini and Luciani ('11) found no 

 constant histological differences between normal and castrated 

 dogs, sheep, domestic cattle, rabbits and Guinea-pigs. In 

 domestic fowls, however, they found, after complete castration, 

 decided changes. There was more or less increase in weight, 

 but hyperaemia was not constant. In the normal fowl they 

 found a relatively small number of eosinophiles. In the capons 

 the eosinophiles had not changed in number, but in addition 



