458 WILLIAM H. F. ADDISON 



Most investigators have found hypertrophy of some type of 

 cell. The majority call it eosinophile (acidophile) . In the 

 rat, I have considered it the basophilic type and that the new 

 basophiles have come from the reserve cells. Kon, in his human 

 case, thought it was the chief cells (reserve cells) which he saw 

 enlarged, and this seems similar to what I have seen in the rat. 

 Marrassini and Luciani, in the domestic fowl, describe the en- 

 larged cells as having "granular protoplasm, stained funda- 

 mentally by hematoxylin and containing a variable number of 

 globules of different sizes, strongly stained by eosin. Between 

 these elements and others of the hypophysis, there are inter- 

 mediary forms which make one think of the possibility of re- 

 ciprocal transformation." From this description, it would seem 

 that the castration changes in the domestic fowl may be some- 

 what similar to those in the albino rat. 



Several find droplets of a secretion appearing in the enlarged 

 cells. Thus Kolde in the rabbit, found little red granules which 

 he thought might be colloid formation, and Marrassini and 

 Luciani in the domestic fowl found the enlarged cells con- 

 taining a varying number of globules. These we have seen in the 

 enlarged cells of the rat hypophysis, and believe the large vacuoles 

 which appear so strikingly in the basophiles to be filled wiih. an 

 accumulation of the same substance. It is a colloid-like secre- 

 tion, we infer, because of its similarity in appearance to the 

 homogeneous colloid substance which appears in the lumen of 

 the pouch of Rathke at the same time. i 



Zacherl's results ('12) on the white rat show the same early 

 changes which we have described, although his interpretation is 

 somewhat different. He looks upon the cells which enlarge as a 

 new cell type 'castration cells' and does not attempt to trace 

 their development. 



Thus, in the various animals examined, even allo\\dng for the 

 several techniques employed, it would appear that the act of 

 castration does not produce the same reaction in the hypophysis. 

 It is generally believed, however, that the balance of the endo- 

 crine glands may be different in different animals, and the results 

 of this form of experiment (removal of one of them) lend support 

 to this view. 



