472 DAVID H. DOLLEY 



volved, it seems safe to deduce that a mild chronic depression, 

 never reaching such an extreme degree, would end the same way. 



The earUer conclusion that karyorrhexis is not flatly degenera- 

 tive (Dolley, '13) is confirmed by the early recovery of the 

 amphinucleolus. 



The changes which unless interrupted proceed to cell death 

 are: a shrunken, homogeneous, eosinophilic and hyaline-like 

 plasma. Corresponding to this is a likewise shrunken hyper- 

 chromatic nucleus, if in the Hodge stage of normal shrunken 

 nucleus, still more shrunken. So far it would maintain the 

 relative excess of nuclear materials. If more advanced, solution 

 of the nuclear membrane follows, and the nucleus is no longer 

 orga;Qically isolated from the cytoplasm, though th'e presence of 

 chromatic substance proves the existence of nuclear material. 

 The last stage is the loss of this chromatic factor, and the cell 

 is reduced to a hyaline-like remnant. From any one of these 

 varied states, then, more or less recovery is possible. 



The decrease of chromatic substance 



It must be remembered further that tinctorial methods do not 

 demonstrate even at the last point the complete disappearance of 

 nuclear material in the shape of nucleolar substance, an acid re- 

 action in common with the plasma. To preclude the possibility 

 of some recovery, the anucleated appearance must stand for 

 an absolute reality. This consideration possibly explains some 

 states of least possible restoration. For example, formless rem- 

 nants of cells appear in all recoveries without any perceptible 

 localized nucleus, yet containing scattered basichromatic granules. 

 The tinctorial reaction of these granules, if paler, suggests a basis 

 of nucleolar substance, that is, their usual composition. This in 

 one way presents no difficulty for the suggestion has been 

 made elsewhere that the extranuclear chromatic synthesis may 

 happen within the plasma (Dolley, '13). The potentiahty of 

 further recovery, however, involves the same problem of nuclear 

 relocalization that exists in less atrophic cells with loss of the 

 nuclear membrane, and will be discussed under the latter 

 phenomenon. 



