518 



NAOKI SUGITA 



TABLE 2— Concluded 



III. TECHNIQUE 



Fixatio7i 



For the exact measurement of the thickness of the cerebral 

 cortex, it js desirable to have material which has suffered the least 

 possible change in volume as result of fixation and also to employ 

 a uniform technique. As a preliminary, I went over King's work 

 ('10) on the effects of various fixatives on the adult albino rat 

 brain, applying several kinds of fixing fluids to brains in differ- 

 ent stages of growth, and imbedding them in parafiine. These 

 brains were sectioned and the cell pictures studied under the 

 microscope to determine the influence of the several fixatives 

 upon the cells and the surrounding tissues. War conditions pre- 

 vented the use of celloidin and photoxylin as imbedding media 

 to be compared with parafiine. 



