520 



NAOKI SUGITA 



TABLE 4 



Showing the average age, body weight, body and tail lengths and brain weight grouped 

 by brain weight of the albino rats used in this study for horizontal sections, 

 accompanied by the calculated standard age, body weight, body and tail lengths 

 corresponding to the given brain weights 



I was convinced that, for the present study, the latter should be 

 exclusively used (cf. also Allen, '16). 



From an examination of the cell bodies and nuclei, I have 

 concluded that they were not much modified by either swelHng 

 or shrinkage. The sections fixed with Bouin's fluid are, however, 

 less well stained with Nissl's soap-methylene-blue-solution, 

 toluidin blue or thionine than the sections fixed with formalin or 

 alcohol. But, if .carbol-thionine solution (1 gram thionine pow- 

 der dissolved in 99 cc. of 0.5 per cent aqueous solution of car- 

 bolic acid) be used as the stain, this defect is remedied, and in the 

 rat brain at least, tl^ey stain distinctly. 



As a result of these preliminary tests the sections used for this 

 study were all prepared by the methods just described and in doing 

 this, the procedure outlined in the next section was followed. 



