70 W. M. SMALLWOOD AND RUTH L. PHILLIPS 
questioned the results obtained by Hodge and Crile. Our work 
was begun in 1910-11, but the opportunity for completing it 
did not present itself until this summer. We have re-examined 
our earlier work and supplemented it with additional material 
collected and prepared in the same way as that obtained 
previously. 
This material consists of the following stages covering the life 
cycle of the honey bee. 
1. Recently hatched larvae. 
. Half-grown larvae. 
. Fully-grown larvae. 
. Early pupae. 
Mid-pupae. 
. Late pupae. 
. Newly hatched adults. 
. Young adults taken at 6.30 a.m. 
. Young adults taken at 6.30 p.m. 
10. Old adults taken at 6.30 a.m. 
11. Old adults taken at 6.30 p.m. 
12. Adults taken at close of the winter season. 
Several different fixatives were tried, but the only ones found 
successful were osmic sublimate, 1 per cent osmic acid, 1 per: 
cent glacial acetic, and sublimate to saturation, Carnoy’s and 
OhlImacher’s fluids. Only one individual, that one of stage (8), 
included in our study was fixed with osmic sublimate. 
No attempt was made to dissect out the brains of the larvae, 
which were embedded entire. The brains of pupae and adults 
were excised. Sections were cut from four to seven micra thick 
in paraffin of 54°, and stained in iron haematoxylin with Bordeaux 
red as a counter stain. 
The Zeiss and Leitz eyepiece micrometers were used, readings 
being computed in micra. We tried to use the planimeter in 
our work this summer, but found it impracticable in measuring 
such small nuclei. 
There are according to Kenyon,® four general regions in the 
brain of the bee; the dorso-cerebron, the ventro-cerebron, and 
COM D TP wd 
No) 
® Journal of Comparative Neurology, vol. 6, 1896. 
