190 



Journal of Comparative Neurology. 



hematoxylin, but remain transparent and highly refractive in 

 preparations double stained with Ehrlich's hematoxylin and 

 Congo red. 



(h.v. rnij- ^ii_ 



Fig. ^. Diagram of Giant Ganglion Cell and its surrounding capsule. «/., 

 nucleus; nil., nucleolus; ch.n., chromatin network partly drawn in; mb.p., 

 membrana prima, «./., neuroglia fibre ; «.«., neuroglia net ; ax., axis cylinder. 



Immediately around the nucleolus the karyoplasm often 

 stains less deeply than in its more peripheral parts. This may 

 be attributed to the karyoplasm in that region being greatly 

 vacuolated. This lightly staining region varies greatly in size, 

 and the definiteness of its outline. The chromatin net show- 

 ing faintly may be followed from the lighter area to the darker 

 area showing the continuity of the karyoplasm. Usually 

 this area is relatively small and its limits indistinct, the 

 denser karyoplasm gradually becoming lighter toward the nu- 

 cleolus. Sometimes it is entirely absent, karyoplasm being ho- 

 mogeneous throughout. This lighter area is often of peculiar 

 and varied shape, sometimes crescentic occasionally sending out 

 forked tongues toward the periphery of the nucleus. In some 

 preparations what corresponds to this area is an empty space, 

 and the karyoplasm can be seen to have shrunken away from 

 the nucleolus at one side leaving a crescent shaped space. 



