THE TRANSPLANTATION OF OVARIES IN CHICKENS 113 
water. Etherized in twenty minutes. Plucked feathers and 
opened body wall between last two ribs. Large ovary completely 
removed or nearly so, in three or four pieces. Hemorrhage slight. 
Stitched in small piece of ovary of no. 11379 to peritoneum near 
attachment of old ovary. Sewed up. Bird recovered rapidly. 
Some Dark Brahma in ancestry, but its characters had become 
eliminated. 
Resuuts, Hxperiment 1. Mated in pen 1027, no. 11605 ° (with 
engrafted ovary from no. 11379, Dark Brahma) and 11291°, 
straight Dark Brahma. Table 1 gives the juvenile character- 
istics of 1, the male; 2, the White Leghorn-Houdan, so-called 
foster-mother; 3, the hen from which the ovaries were transplanted; 
4, expectation on the hypothesis that the graft succeeded; 5, 
expectation on the hypothesis that the graft failed and the proper 
ovary was regenerated; and 6, the observed characteristics of the 
young offspring. 
An examination of table 1 shows at once that it cannot be true 
that the engrafted ovary replaced the hen’s proper ovary, for if it 
had, columns six and four should agree. On the contrary, col- 
umn six agrees essentially with column five and supports the 
hypothesis that the engrafted eggs did not become functional. 
One discordant fact there is, however, namely, the occurrence in 
column six of three cases of cinnamon offspring. Such offspring 
are to be expected on the hypothesis that some eggs of the graft 
became functional. If that hypothesis be true, then the other 
characters of the same individuals should be like those of the 
pure Dark Brahma. Of the three the first has extra toes, split 
comb and a boot of one row; it is no Dark Brahma; the second 
has extra toes, wide nostril and a two rowed boot; it is not a 
Dark Brahma; and the third has really black down with some red 
at the tips, five toes on the right foot, a split comb and one row 
of feathers on the shank; so it is not a Dark Brahma. These 
therefore, are not from the engrafted Dark Brahma eggs. They 
represent cases of imperfect dominance of the black down over 
cinnamon. ‘The conclusion to be drawn from this experiment is 
that the engrafted eggs did not mature in the foster-mother. 
