288 E. A. ANDREWS 
use of the leg of that side of the body, but cases were recorded in 
which the advanced stylet was on the opposite side from the 
crossed leg. Males crossed the right leg with either right or left 
stylet advanced and males crossed the left leg with either right , 
or left stylet advanced. Here again there is the possible objec- 
tion that the condition observed was not permanent or the one 
employed in actual sperm transfer. More minute observation 
of several normal cases are necessary. 
One good case seems, however, rather conclusive. In this a 
male, in November, crossed the left fifth and advanced the left 
stylet, but after an hour of attempts to enter the annulus, crossed 
the right fifth and five hours later the right stylet was one mm. in 
advance of the other and the female had a sperm plug in a right 
annulus. Here the leg and stylet used did coincide, but the 
annulus was not the one to be expected. 
Again in some conjugates killed by boiling while united it was 
found that in one a right stylet was advanced to a right annulus 
and in others a left stylet to a left and to a right annulus. 
As far as the evidence goes it gives the impression that the male 
is free to use either right or left stylet with either right or left 
fifth legs till successful in getting some one tip of the stylets into 
the vestibule of the annulus, which may be a right or a left one, 
indifferently. Yet future observations may show that the lines 
of least resistance are for the male to use the left stylet for the 
right-handed female, and the reverse, and that this actually takes 
place, in nature as the normal, though we doubt if it be at all neces- 
sary. Observations show that both papille are ready to dis- 
charge sperm at the same time and it should be determined by ex- 
periment whether the male uses both right and left sets of sperm 
transfer organs, alternately, at each conjugation or not. 
When the first and the second stylets were cut off from one side 
of some sixteen males and, either at once or some weeks after, 
these males were given females, the unexpected result followed 
that in spite of many repeated attempts, one lasting nine hours, 
the numerous conjugations of these unilaterally mutilated males 
did not result in any clear cases of successful sperm transfer. In 
