By bs CHARLES W. HARGITT 
Itis just at this time that the maturation phenomena are in proc- 
ess of development. In my previous account some doubt was 
expressed as to the presence of mitosis. Critical study of fresh 
material has enabled me definitely to confirm the facts of matu- 
ration mitoses attested by Smallwood (’09) in Hydractinia, and 
Beckwith (’09), in Clava. A critical review of my earlier material 
only went to confirm the previous doubt; all of which but tends 
to resolve the case to one of technique. In the newer material 
both maturation mitoses were distinguishable without serious 
difficulty. 
2. Nuclear behavior. In addition to the foregoing discussion 
some further reference to points of nuclear behavior seems desir- 
able. In several of my earlier accounts attention was directed 
to the migration of the nucleus to the distal periphery of the egg 
as it approached maturity. Asis well known, many earlier stu- 
dents of nuclear physiology have sought to correlate directly the 
nucleus with nutritive’ functions during the growth period, and 
its location has been said to conform to this conception, and nu- 
merous citations made to facts recorded in phyla above protozoa. 
So far as the Hydrozoa are concerned I am not able to confirm 
this view. In the growing oocyte of Clava the germinal vesicle 
is rarely if ever directly contiguous to the nutritive surface of 
the spadix, and in the period of later growth invariably migrates 
to the distal surface and comes to lie in immediate contact with 
the outer wall of the gonophore. While I have not made any 
attempt at this time to take up the problem for critical inquiry 
and investigation, yet my general observations tend to render 
extremely doubtful the view above suggested, at any rate in any 
very explicit and causal sense. That the nucleus may function 
in this matter in a general way as in many other vital functions 
may be probable, yet that its primary or fundamental and direct 
part in the oocyte has to do with nutritive more than other func- 
tions of cell life seems more than doubtful. It may easily be 
shown that processes of nutrition, along with other phases of 
metabolism, are functions of the entire cell working as a whole. 
In the earlier paper attention was directed to the phenomena of 
metabolism as related to the origin and development of the pig- 
