SPERMATOGENESIS OF EUSCHISTUS 767 
THE CHROMATOID CORPUSCLES 
These are the bodies that were called yolk globules in my paper 
of 1898. They are rounded bodies within the cytoplasm, which 
stain in general like the plasmosomes, 7.e., red after Delafield’s 
haematoxylin and eosin, and red after the Ehrlich-Biondi triple 
stain, with which the chromatin stains blue and green respectively. 
But with the triple stain of Hermann they color with safranin, 
while the plasmosomes are pale violet, and with iron haematoxy- 
lin the latter stain intensely black. At the time of their first 
appearance in the first spermatocytes they lie usually near the 
nuclear niembrane (ch. c, figs. 59, 63). Through the prophases 
of the first maturation mitosis they are regularly seen (figs. 65, 
66, 71, 85). During the maturation mitoses they lie outside of 
the spindle and do not divide (figs. 87-89, 92, 98, 95, 99-104, 
107, 109, 111). In the second mitosis it is not uncommon to 
find several of them in one daughter cell and none in the other 
(figs. 104, 107-109), or again they may be found in both, thus their 
apportionment to the spermatids seems to be a matter of chance. 
During the histogenesis of the spermatids they occur in variable 
positions (figs. 111, 112, 115, 116, 117-120.) Later than the 
stage of fig. 124, which shows one close against the nucleus, I 
have not been able to see them. 
The name ‘chromatoider Nebenkérper’ was introduced by 
Benda (91) for similar bodies in mammalian spermatocytes. 
They have been more fully treated especially by Lenhossék 
(98), the Schreiners (’05, ’08)-and Meves (’99). The Schreiners 
have reviewed the literature, and find that in Myxine the chro- 
matoid corpuscle is produced by the fusion of several ‘chromatoid 
nucleoli’ (distinguishable from paler nucleoli) that emigrate from 
the nucleus of the first spermatocyte; this corpuscle persists in 
the cytoplasm into the spermatid, and in the latter eventually 
wanders back into the nucleus. They were the first to have 
described its nuclear origin and nuclear return, while Meves had 
previously (’02) suggested they might be discharged plasmosomes. 
