TRANSPLANTATION OF LIMBS IN AMBLYSTOMA 131 
duction; 2) flexion and extension of the forearm; 3) flexion and 
extension of the carpus, and, 4) movements of the digits. When 
the transplanted limb showed inability to carry out all of these 
movements such as occur in the normal intact limb, it was placed 
in the category, ‘function impaired’ (table 1). 
The functional behavior of the transplanted limbs in all series 
was closely observed and recorded, these observations being 
TABLE 1 
A. Showing the results of transplanting the right anterior limb rudiment a given 
number of segments (AS1, AS2, AS3, etc.) posterior to its normal position 
B. Showing the results of transplanting an additional anterior limb rudiment to 
an embryo in which the normal limb rudiments have been left intact. HS3, HS4 
and HS65 indicate the successive number of segments the additional limb rudi- 
ment was implanted posterior to the normal right anterior limb of the host 
NORMAL REDUPLI- | FUNCTIONAL NO FUNCTION | FUNCTION 
LIMBS CATIONS LIMBS FUNCTION IMPAIRED PERFECT 
a POSITIVE 2 SS ee) |S See SS 
SERIES Nae es ay : E g X g : g is g : E 
Boe Nabeec bee NP omrealice |oheshil tet Mieasenbean | (a 
io) Ay ie) ew ie) Ay 1s) Ay ie) Ay is) Ay 
A 
ASI 25 20 | 19 195.0; 1 | 5.0] 20 |100.0/ 0 | 0| 115.0] 19 195.0 
AS2 A 18 | 17 |94.5] 1| 5.5117 [100.0 0 | 0| 3 |16.7] 15 183.3 
AS3 24 17 |-13 |76.5| 4 |23.5] 15 | 88.2] 2 \11.8] 4 |23.5] 12 164.7 
AS4 34 29 | 18 |67.0] 11 |33.0| 23 | 79.3] 6 [20.7] 11 [37.9] 12 |41.4 
ASS 35 30 | 11 [36.7] 19 163.3] 20 | 66.7] 10 |33.3] 16 [53.3] 4 |13.4 
AS6 35 | 29 | 22 |75.9| 7 [24.1] 15 | 51.7| 14 [48.3] 15 [51.7] 0| 0 
: B 
HS3 10 9 | 2 |22.2| 7 I77.8| 9 |100.01 0| 0| 6 \66.7| 3 (33.3 
HS4 30 21 | 8 |38.1] 13 [61.9] 11 | 52.4] 10 [47.6] 11 [52.4 0 | o 
HS5 30 25 | 11 [44.0] 14 [56.0] 13 | 52.0] 12 [48.0] 13 [52.0 0 | 0 
frequently made from a period when normal limb reflexes first 
began to appear until preservation of the animal. The method 
employed in studying the limb reflexes consisted in the temporary 
transfer of the animal from the individual aquarium (battery 
jar) in which it was reared, to a small receptacle, such as a watch- 
glass. In this way the animal could be brought under direct 
observation either through the use of a binocular microscope or 
