TRANSPLANTATION OF LIMBS IN AMBLYSTOMA 145 
muscular differentiation, in addition to a typically developed 
shoulder-girdle. 
In general, however, the defective function of limbs in this 
position cannot be regarded as a result of factors already con- 
sidered, but it is conditioned by other factors, which will be con- 
sidered later. The completeness of function is In no way corre- 
lated with the time in days after the operation when reflexes 
first begin to appear. For example, in case AS5.; limb reflexes 
began twenty-two days after the operation, while in some other 
cases with impaired function reflexes began as early as seventeen 
days after the operation. 
Series AS6. Limbs transplanted six segments posterior to 
the normal position so that they centered ventral to the tenth 
somite were so far removed from the normal situation that there 
were no cases which functioned perfectly. Although 51 per cent 
were capable of some movement, in all cases the movements 
were greatly restricted (table 1 and fig. 8). Case AS69 of this 
series (fig. 20) was the only one in which were observed move- 
ments codrdinated with those of the opposite intact limb, yet 
these movements were very restricted, especially movements of 
the arm on the shoulder. The segmental nerve contribution 
to the limb is shown in table 2. 
In case AS6e, all of the shoulder muscles were differentiated 
and all but three received peripheral innervation from a plexus 
composed of the eighth, ninth, and tenth segments of the cord. 
An examination of table 2 shows that peripheral innervation 
in this case was as complete as in limbs of the series AS5. The 
shoulder-girdle also was quite well developed and showed no 
more defects than in those not so distantly removed from the 
normal situation. 
It has hitherto been observed (table 1) that there occurred a 
general increase in the number of reduplications as the limbs 
became transplanted farther and farther away from the normal 
situation. This, however, was not the case with series AS6, 
in which only 24 per cent developed reduplication, which is in 
marked contrast with the 63 per cent which reduplicated in 
series ASS. The small number of reduplicated appendages of the 
