186 M. F. GUYER AND E. A. SMITH 
Experiment 22 
Tnasmuch as it was desirable to get new defective-eyed indi- 
viduals from a stock wholly unrelated to the line in which we 
had originally produced eye defects, and since 16A1, after treat- 
ment with lens-sensitized serum, had already given us an indi- 
vidual with conspicuously abnormal eyes (opaque lenses and re- 
duced size), it was determined to try her again. The fowls used 
were two which had been left over from the earlier experiment in 
which 16A1 had been used (table 5), and as they had had their 
70A/ TOAR 3 10A3 
Fig. 2 Female 16A1 was mated to her brother, 16A2, shortly after which she 
was treated (table 5) with lens-sensitized fowl serum; one of her young, a male, 
had both eyes abnormal. See figure 5 for explanation of symbols. 
Fig. 3 Female 16A1 was mated to male 50 and subsequently treated with 
lens-sensitized fowl serum (table 6); two of the resulting young, 70A1 and 70A2, 
each had both eyes defective. For explanation of symbols see figure 5. 
last injection of pulped rabbit lens on March 26th it was thought 
advisable to resensitize them. They were given three addi- 
tional doses of lens intraperitoneally on June 12th, 14th, and 
20th, respectively (table 6). They had in the previous sensitiza- 
tion (table 5) been given two of the injections of lens intrave- 
nously. 
Rabbit 16A1 was the daughter of a female shipped from Min- 
neapolis and was, therefore, unrelated to our Madison stock. 
She was mated to no. 50, a male obtained in Chicago. On the 
eighth, tenth, fifteenth, and seventeenth days of pregnancy, re- 
spectively, she was injected with 4 cc. of the lens-sensitized fowl 
serum through the marginal vein of the ear. On July 21st five 
