192 M. F. GUYER AND E. A. SMITH 
Experiment 19 
Inasmuch as only one rabbit was used and no young were 
born, the experiment (table 12) is not significant beyond helping 
to complete our record and also to demonstrate some of the dif- 
ficulties and discouragements which attend this kind of work. 
Experiment 23 
With this experiment (table 13) the use of fowl serum sensi- 
tized to rabbit testis was begun. Four fowls and four rabbits 
were used. The fowls were given four injections of pulped testis 
TABLE 12 
Experiment 19 
Control: Treatment of rabbit with normal serum 
IDENTIFI- 
DATE OF pe NUMBER bear sige DOSE OF SERUM REMARKS 
RABBIT 
cc. 
September 26 13 9 6 Mating, 138 2 xX 44c¢ 
September 28 13 11 5 + 3 (normal salt) 
October 1 13 13 5 + 3 (normal salt) 
October 2 13 14 6 No young 
at intervals of about a week. To prepare the injection mass the 
testes of two adult rabbits were pulped by grinding in a mortar, 
normal saline solution being poured in from time to time until a 
total of 18 ec. had been added The mass was then pressed 
through two layers of cheese-cloth to strain out the larger par- 
ticles which would occlude the cannula of the syringe. Unlike 
lens emulsions, such emulsions of testis are always tinged more 
or less with blood. This would lead one to expect a more severe 
hemolytic reaction from antiserum produced from such emul- 
sions than from normal serum or fowl serum sensitized to such 
relatively bloodless tissues as the lens or the humors of the eye. 
Whether, in fact, an increased intravenous hemolysis occurred in 
the rabbits treated with serum sensitized to testis we did not 
