FERTILIZATION IN THE HONEY-BEE 2a 
arrangement seems to be due to the fact that the nuclei bulge 
the walls slightly outward, and force nuclei of adjacent cells 
alternately upward and downward. The chromatin of the nuclei 
is mostly in three to five granules, the rest faintly scattering 
through the clear plasma. The chromatin stains more densely — 
while secretion is taking place, and shrinks and takes the stain 
less densely when it ceases, but its disposition in the nucleus 
does not appear to alter. The cytoplasm is very finely granular 
(after fixation), slightly more densely staining around the nuclei, 
especially when the cells are functionally active after growth 
is complete. The distal ends of the cells contain larger and 
denser staining granules that give, in (cross-) section of the epi- 
thelium, the appearance of a dense granular band (pl. 2, figs. 
5a, and 6a). 
The cells of the glandular epithelium are modified according 
to the region of the lumen which they line. The cells lining 
the vas deferens between testis and seminal vesicle (text fig. 1, f) . 
and those between the vesicle and the mucous gland (h) are more 
cubical than columnar. Here the nuclei are placed more evenly 
side by side and have a more founded outline, but the character- 
istic structure and mode of secretion of the.cells is identical. 
The cells of the seminal vesicle are about a half shorter than those 
of the gland, their nuclei are smaller and similarly disposed. 
Secretion takes place by strangulation, with dissolution of the 
cell substance (pl. 2, figs. 5 and 6b). The dense granular area 
at the tip of the cell widens, the granules increase in size, in 
refractiveness, and in density of staining, and finally vacuoles 
may appear among them. The end of the cell rounds up into 
a globule of secretion, which sloughs off into the lumen of the 
organ. ‘This process is most pronounced in the gland, where 
the secretion retains its coarse granular character. These 
granules are transformed to highly refractive globules of some- 
what larger size, as if by absorption of some of the fluid; the mass 
of the secretion at the same time becomes more viscous. In 
the seminal vesicle the granules are smaller and soon dissolve 
to a pale plasma (pl. 2, figs. 5 and 5b). In the narrow portions 
of the vas deferens, at either end of the vesicle, neither the granu- 
