FERTILIZATION IN THE HONEY-BEE 245 
3. The ejaculatory duct. The ejaculatory duct and the glandu- 
lar lining of the basal portion of the gland with which it connects 
remain to be described (pl. 3, figs. 7 to 10). The blind end of the 
duct penetrates the gland’s muscular coat; here it expands into 
a cone whose base becomes applied, as described before, to that 
aspect of the gland’s basal region which is directly opposite the 
opening of the vas deferens. The hypodermal cells forming 
this cone become elongated around its base from cubical to a 
distinctly columnar form. ‘The base of the cone becomes heavily 
chitinized, especally that part lying over the cells which are 
most elongated (pl. 3, fig. 9). At the center of the base the 
cells are shorter, and here the chitin is laid down in two layers 
over a very small area (pl. 3, figs. 7 to 10, n). Between these 
two layers is a small mass of material staining more densely 
(with iron-alum-haematoxylin) than the chitin, which later 
disappears or else shrinks greatly, leaving the two layers separated 
by a space. The layer toward the lumen of the gland is con- 
siderably thinner than the other (pl. 3, fig. 10), n); both together 
they form a weakened area in the base of the cone which may be 
likened to a drum. 
The columnar cells of the base of the cone now recede laterally 
and decrease in length, finally leaving this double chitinized 
drum alone to close the end of the duct (pl. 3, figs. 9 and 10). 
At the same time the glandular layer of the mucous gland breaks 
down as described heretofore, and these gland cells over the 
center of the chitinized drum also withdraw laterally. This 
leaves the chitin drum exposed to the lumen of the gland, but 
unperforated, in which condition it can be demonstrated at all 
stages investigated, provided methods are used in killing which 
do not distort the organs to such an extent that the drum is 
burst. This fact, together with evidence to be submitted, indi- 
cates that both sperms and mucus remain in the seminal vesicle 
and gland, respectively, not only until maturity, but even until 
copulation. 
