AUTHOR’S ABSTRACT OF THIS PAPER ISSUED 
BY THE BIBLIOGRAPHIC SERVICE, APRIL 19 
FERTILIZATION IN THE HONEY-BEE 
Il. DISPOSAL OF THE SEXUAL FLUIDS IN THE ORGANS OF THE 
FEMALE 
G. H. BISHOP 
Zoological Laboratories of the University of Wisconsin 
TWO TEXT FIGURES 
CONTENTS 
rat OCU ETO MMs Pepe et ATTEN Lae ie ere ee ae eds ees ole eis Sate hate ceeeekdes 267 
ERESHORIC ANS Air tune ee te ae ALE AS de ee wale Se oie gehen 268 
Amat OMY Ol SOXUa lap PALALUS ic < Scla cine elens) have Usos wa idw abides aged Hors Oeraes 269 
DEseniptionzOf lent wliZeGe Queens! nec nis wie ow kyoes ,<o arse meme eee soe eye oe neers 278 
SUMiMnahy Andy COMEMATONSS a... cc .2aae Sead Mh ees AER lace ae een ae 282 
INTRODUCTORY 
In the paper preceding this (Fertilization in the Honey-bee, I) 
has been described the sexual mechanism of the drone bee and 
its physiological functioning in copulation. As a check to this 
work, a series of dissections and microscopic examinations has 
been made of queen bees which had flown from the hive and 
been mated under entirely normal conditions. Eight virgin 
females were watched and their time of mating recorded, and 
were subsequently examined to determine the behavior and the 
disposal of the sexual secretions of the drone in and after 
copulation. 
These queens were killed for examination at specific intervals 
of time after their mating, and examined either fresh or after 
preparation for histological study, as conditions appeared to 
render desirable. The time data were obtained in the following | 
manner. The virgins were introduced into strong nuclei of 
young bees, and these nuclei were inspected every half-hour 
during the warm bright part of the day, beginning in each case 
when the virgins were four days old. The bees showed little 
effects of handling, and the fact that queens were mated from 
267 
