280 G. H. BISHOP 
The penis attached to this queen was distended with white 
mucus to a point slightly beyond the tips of the queen’s last 
segments. It was fixed with the queen; then dissected out, and 
an attempt made to section it. But either the fixative used 
(Gilson’s) or else exposure to the air had so hardened the mucous 
content as to nick the microtome knife and destroy most of the 
significant sections. Several points could be made out: first, 
that the mass contained mucus and few, if any, sperm; second 
the bulb of the penis was not everted (fig. 2, B) (though the organ 
was firmly attached to the queen), and, third, the mucus was 
contained in the bulb of the penis and in the enlarged end of the 
ejaculatory duct adjoining it. The sheath of the penis surround- 
ing the uneverted bulb was torn from the bulb in removing the 
organ, exposing the bulb and ejaculatory duct. In this case, 
therefore, these parts served analogously to a spermatophore, 
except that the contained fluid was mucus without sperm, all 
the sperms having apparently been forced on into the queen’s 
oviducts. 
C (mated not over one and one-half hours). This queen was 
inspected at once. Both oviducts were distended with sperm 
and mucus, still uncoagulated (until exposure), and the sperma- 
theca contained many sperms in a clear medium. ‘The penis 
attached in the vagina was shrunken considerably and had ap- 
parently been extruded completely with eversion of the bulb, 
as in extrusions produced experimentally (previous paper, p. 250). 
It contained but a small amount of mucus, no sperms, and it 
was torn off close behind the bulb. 
D (same time as C). This queen had already lost the drone 
organ. Histological sections showed in general the same con- 
ditions as obtained in B, but both oviducts were distended, and 
yellow in the fresh condition as before. Sperms had scattered 
somewhat through the mucous mass, but were mostly still un- 
mixed with the mucus, and most of them were gathered along 
the oviduct walls, especially dorsally. The mucus appeared less 
densely staining and showed evidence of solution along the edges 
of the mass, except that in the posterior region of the vagina 
the mucus stained intensely, as mucus does which has been 
