332 EDWARD F. ADOLPH 
IT. Constant conditions. In these experiments etherized flies 
were placed within glass bottles or vials, which were closed by 
perforated filter-paper caps or by loose cotton plugs. The num- 
ber of female flies in a single container varied from 1 to 100. The 
bottles were exposed to the atmosphere of the laboratory room. 
1. Controls. The simplest conditions obtainable gave a slight 
amount. of egg-laying. When the flies were penned in dry glass 
containers, they laid an average of 0.05 egg per day. When 
moisture was added by saturating cotton with distilled water, 
a higher average, 0.18, was obtained, as shown in table 2. 
TABLE 2 
Non-selective expermments 
| AVERAGE 
KIND OF STIMULUS STIMULATING SUBSTANCE leat ea, S eaaend NO. CAGES 
PER FLY 
I. Moistures? . ..: none (glass) 0.05 Ag 605 54 
water 0.18 48 200 12 
2. Texture........| boiled agar ORM 209 274 33 
boiled starch 0.26 13 25 5 
amphibian jelly 0.17 1 6 2 
So. plastee. so. ne. Telucose 0.20 19 32 4 
sodium chloride 0.08 Y 8 1 
ANOdORe ee ie acetic acid + alcohol 0.06 2, 200 3 
formic acid 0.04 13 70 7 
acetic acid 0.02 3 50 10 
butyl! alcohol 0.006 1 ie) 8 
fermenting banana 0.005 2 130 26 
5. Taste + odor....| ethyl ether 0.21 D 8 1 
ethyl aleohol 0.16 A5 106 ily 
acetic acid 0.10 25 50 10 
chloroform 0.08 2 8 1 
methyl! alcohol | 9.05 20 100 10 
The following stimulating substances called forth no responses: 
1. Dry starch, bismuth subnitrate, zine stearate. 
2. Boiled gelatin, chalk + wet cotton, wet clay, wet bismuth subnitrate. 
3. Sucrose, glycerin. 
4. Ethyl sulfate, ethyl oxalate, isoamyl acetate, amyl valerianate, allyl alco- 
hol, amyl aleohol, isoamyl alcohol, tertiary amy! alcohol, propy! alcohol, iso- 
butyl alcohol, tertiary butyl alcohol. 
5. Acetic acid + alcohol. petroleum. 
