134 



A. FRANKLIN SHULL AND SONIA LADOFF 



TABLE 5 



07ie of the two lines here recorded was reared in spring loater, the other in a solution 

 of calcium chloride more dilute than that used in table 4- Again there were more 

 male-producers in the calcium chloride 



* Remainder of family not recorded. 



of rearing it continuously in one medium, alternate generations were 

 reared in spring water and a ^^^^ solution of calcium chloride. All 

 members of one family were reared, from the time they were isolated 

 shortly after hatching, in the same medium. That member of each 

 family which was selected to become the parent of the next generation 

 laid her eggs in the medium in which she was bred, and her eggs hatched 

 there. But shortly after hatching, her daughters were transferred 

 to the alternate medium, and there reached maturity. 



Most of the male-producers appearing in this experiment, 

 as shown in table 6, were offspring of females that lived and 

 laid their eggs in calcium chloride solution. Since it is the growth 

 and maturation period of an egg in which it is determined whether 

 a male-producer or a female-producer will develop from that 

 egg, as shown by the earlier experiments of ShuU ('12), the results 

 given in table 6 are what would be expected if calcium chloride 

 favors male-production. In this respect Experiment 6 is in 

 accord with the other calcium chloride experiments above. 



