380 



RALPH S. LILLIE 



TABLE 3 



Experiment of August 19. Eggs were fertilized at 11.04- «• w. and placed in dilute 

 sea water (60 per cent dilution) at the following intervals after fertilization 



TIME OF PLACING IN DILUTE 

 SEA-WATER AND CONDITION 

 OF EGGS AT THAT TIME 



EFFECT OF DILUTE SEA-WATER ON EGGS (CONDITION AFTER 

 3 AND 30 MINUTES) 



10 



11 



12 

 13 

 14 

 15 

 16 



2 m. (all with fertili- 

 zation membranes). 



4 m. (unchanged). 



6 m. (unchanged). 



8 m. (unchanged). 



40 m. (eggs unchanged). 



42 m. (unchanged). 



44 m. (unchanged). 



46 m. (still round and 



unchanged). 

 48 m. (cleavage-f u r - 



row beginning in a 



few eggs). 

 50 m. (furrow visible 



in about half the 



eggs) 

 52 m. (most eggs 



cleaving). 

 54 m. (90 per cent or 



more in 2-cell stage). 



56 m. (all in 2-cell 

 stage). 



58 m. (all in 2-cell 

 stage). 



60 m. 



62 m. 



3 m. : all eggs swollen but intact, many extra- 

 ovates. 



30 m.: all eggs intact; 30 to 40 per cent extra- 

 ovates. 



3 m. and 30 m. : all eggs intact, but few extra- 

 ovates, ca. 1 per cent. 



3 m. and 30 m. : all intact; almost no extra- 

 ovates. 



3 m. and 30 m. : all intact. 



3 m. : all intact. 



30 m. : nearly all intact; 1 to2per cent cytolyzed. 



3 m. : all intact. 



30 m. : nearly all intact; ca. 4 to 5 per cent cy- 

 tolyzed. 



3 m. : nearly all intact; a few cytolyzed. 



30 m. : ca. 10 per cent cytolyzed; the rest intact. 



3 m. : ca. 20 per cent cytolyzed; the rest intact. 



30 m. : ca. 35 to 45 per cent cytolyzed. 



3 m. : ca. 50 per cent cytolyzed. 



30 m. : great majority cytolyzed (ca. 80 to 85 pei 

 cent). 



3 m. : ca. 65 to 70 per cent cytolyzed. 



30 m.: 90 per cent or more cytolyzed. 



3 m.: great majority cytolyzed. 



30 m.: almost all cytolyzed (ca. 95 per cent). 



3 m. : ca. 35 to 45 per cent cytolyzed. 



30 m.: now many intact eggs; 60 to 70 per cent 

 cytolyzed. 



3 m. : two-thirds or more intact. 



30 m. : most eggs intact; 25 to 35 per cent cyto- 

 lyzed. 



3 m.: great majority intact. 



30 m. : great majority intact; ca. 20 to 25 per cent 

 cytolyzed. 



3 m. : ca. 90 per cent intact. 



30 m. : great majority intact; ca. 10 to 15 per cent 

 cytolyzed. 



3 m. : nearly all intact. 



30 m. : nearly all intact; ca. 5 per cent cytolyzed. 



