414 E. p. CHURCHILL, JR. 



that the experiments should continue. The cytoplasm was 

 studied after staining with eosin or Bordeaux red with the same 

 results as in the previous experiments. The cytoplasm of those 

 mussels which had been in the albumin solutions was more 

 granular, the granules being more numerous and larger, than in 

 the mussels which had remained in water only. This was 

 especially apparent in the cases of two pairs of mussels from 

 an experiment extending over six days. The solution in which 

 the mussels had been kept contained 100 cc. of albumin in 5000 

 cc. of water. The cytoplasm was stained, in the case of one 

 pair of mussels, with eosin and in the other with Bordeaux 

 red. In both cases there were more granules in the cytoplasm 

 of the mussels which had remained in the solutions than in that 

 of the others. If the presence of a greater quantity of granules 

 in the cells of the mussels which had been in the albumin solu- 

 tions may be taken as evidence of the presence of albumin, in 

 these cases in which the mouths of the mussels were plugged 

 the albumin could have been taken up only by direct absorption 

 from the solution. 



Considerable effort was expended in attempting to discover 

 a specific stain for the albumin, so that its presence might be 

 detected in a manner similar to that by which fat is revealed by 

 means of Sudan III. Albumin was precipitated with absolute 

 alcohol, caught on filter paper and mounts made of it, both 

 while wet from the alcohol and also after dehydrating, clearing 

 and embedding in paraffin. WMle unstained, the appearance 

 of the albumin under the microscope was that of a quantity 

 of granules of various sizes. Most of them were irregular in 

 shape but now and then one of a spherical form was discernible. 

 Some of the mounts were carried through one stain, some through 

 another, the object being to find a dye that would stain albumin 

 and yet not stain the general cytoplasm of a cell. No great 

 degree of success was attained. The albumin took the various 

 stains tried, including eosin, Bordeaux red, haematoxylin, Janus 

 green and others. All of these dyes stain the cytoplasm of 

 fixed cells except haematoxylin, which is not considered a cyto- 

 plasmic stain. Since, however, the only color this stain im- 



