REACTION OF EMBRYONIC CELLS TO SOLIDS 



531 



In the cultures mounted in plain drops the tissue was found in 

 two cases to be in contact with the cover-slip, upon which a few 

 cells wandered out from the main mass (fig. 3). A small number 

 of nerve fibers were seen growing out upon the cover in one case. 

 None of the others showed any cell movement. It is clear from 

 the experiments that the inorganic media are not so favorable 

 for the cultivation of tissues as plasma or serum.^i 



Table 2 gives a summary of the experiments with frog tissues. 



The general character of the different types of culture is shown 

 in the figures. Figures 1 and 2 represent two preparations from 



TABLE 2 

 Summary of results of experiments with frog tissues 



CHARACTER OF CCI-TURE 



Defibrinated serimi 



Spider web 



Large drop 



Spread drop 



Saline solutions 



Spider web 



Large drop 



Spread drop 



NUMBER OF 

 PKEPARATIONS 



17 



11 



5 



251 

 5 

 5 



CELL WANDER- 

 ING OCCURRED 

 ON SOLID 

 SUPPORT 



15 



1 



11 

 2 



NO CELL 

 WANDERING 



2 

 11 



4 



14 



42 



3 



PER CENT 



POSITIVE ON 



SOLID 



SUPPORT 



20 

 44 

 40 



1 Exclusive of three cases in tap water in which disintegration began very early. 



2 In one case tissue touched bottom of chamber and some outwandering oc- 

 curred. 



Series III mounted respectively upon spider web and in a plain 

 drop. In the latter (fig. 2) there are a great many loose cells 

 which have separated from the main mass of tissue and assumed 

 spherical shape, while the main mass itself looks to be upon the 

 point of disintegration, though, as subsequent observation showed, 

 this did not take place. The drawing was made about twenty- 

 eight hours after the tissue was prepared. In figure 1 the com- 

 panion preparation is represented. The mass of tissue is held 

 against the cover-slip by the spider web and is much more flat- 



21 Cf. M. R. and W. H. Lewis, Anat. Rec, vol. 5, 1911. 



