210 LORANDE LOSS WOODRUFF 
B. RESULTS 
A Series. The A series gives evidence as to the general influ- 
ence of the atmosphere as a source of protozoan life in labor- 
atory infusions. The mouth of the containing flasks measured 
13 inches in diameter and thus afforded ample exposure to the 
air without rendering rapid evaporation troublesome. The 
flasks stood during most of the time in a room in which hay was 
being used for various purposes, and consequently there was 
ample opportunity for the air to be contaminated with cysts, ete. 
Also, during the day time the windows at either end of the room 
allowed a considerable current of air to pass over the flasks. Again, 
certain flasks were placed on a shelf outside of the window where 
they were exposed to the air of the campus. 
The results derived from this series are as follows: Ayl 
remained free from protozoa from the start to October 31st, at 
which time it was seeded with Paramaecium and Oikomonas. 
Two days later it showed a good growth of each of these forms, 
thus proving that it was a favorable fluid for protozoa. Ay2 was 
sterile in regard to protozoa until September 24th when a very 
few tiny amoebae appeared, and remained until October 31st, 
when the culture was destroyed. At/ contained on August 26th 
a few small hyaline bodies which seemed to be cysts. A week 
later there appeared a few tiny amoebae, and on September 
24th a heavy growth of monads was observed which persisted to 
the discontinuance of the infusion on October 31st. At2 remained 
sterile until September 24th when tiny amoebae appeared and 
continued to be present to the end. On October 31st the infusion 
was seeded with paramaecia and these had greatly increased in 
number by November 2nd when the infusion was destroyed. 
At3, which was kept plugged as a control, was first examined on 
September 2nd and was sterile. It was discontinued at this time. 
The At4/ and Ay4 cultures were seeded at the beginning with 
Paramaecium and Oikomonas and showed heavy growths from 
the start—thus proving that the media, from the inception of the 
experiments, offered favorable conditions for protozoan life. 
These two infusions were discontinued on November 2nd. 
