248 LORANDE LOSS WOODRUFF 
Hypotrichida. ‘These forms appeared in negligible numbers at 
the bottom in groups II and IIT, while in group I the largest 
bottom count was taken, i.e., 110 per ce. on the 33rd day (cf. 
fig. 14). Group IV also showed a relatively large bottom count 
as compared with the top count, having a maximum of 80 per ce. 
on the 24th day. 
Paramaecium. B I showed a larger number of Paramaecia at 
the bottom than at the top. Conjugating specimens were seen at 
the bottom only (ef. figs 5 and 12). B II showed practically no 
paramaecia at the bottom, but this is explained by the fact that 
Didinium early exterminated them in this group. A heavy 
growth appeared in B JI/ after conjugation was prevalent at the 
top (ef. fig. 13). B IV had very few paramaecia at the bottom 
until the 34th day and then there appeared about 500 per ce. 
All disappeared by the 45th day. Their appearance at the bot- 
tom was coincident with a decline at the top which was brought 
about by Didinium. 
Vorticella. In group I this form attained a maximum of 175 
per ce. on the 38th day, while in group II they reached a maximum 
of 240 on the 20th day. Vorticella appeared in group III in 
small numbers at both top and bottom, the bottom maximum 
being 60 per ce. on the 31st day. In group IV, however, the larg- 
est bottom count was recorded, i.e., 600 per ec. on the 28th day 
(clase. 115). 
Amoeba. There was a great difference in the amoeba fauna of 
B1 and B2 of group I, so that it is better to present these separ- 
ately. Bi had a maximum of 10000 per ce. on the 58rd day, 
while B2 contained practically no amoebae at any time. The 
B II group showed a large growth which attained a maximum of 
2500 per ce. on the 50th day and terminated on the 58th day. 
There was a relatively small maximum of 250 per cc. on the 37th 
day in BIIJ and the amoebae had disappeared by the 45th day. 
A maximum of 2000 per ec. was in existence in B IV from the 35th 
to the 45th day, when the last regular observation was made. 
This culture, however, supplied countless Amoeba proteus for 
class use for two years thereafter. 
