CHEMICAL PROPERTIES 
OF HAY INFUSIONS 
TABLE 1 
PRELIMINARY INFUSION 1* PRELIMINARY INFUSION 2* 
aa eee ; i : 
2 a SOLIDS meer a SOLIDS 
Q Zap = =) | Zp =” — =| 
a ee | a- | Be | 
Sl Fos Grams per 100 cc. Bie || 9 aS Grams per 100cc. | 2|& 
mw. | KO infusion Sie ish i) da infusion | aye 
SPP? iz PAS aL Les AES! Z 5/5 
BSa /ES2 | gle |[ES2 |8Se Ae 
jogs jogs 4\z |o48 |e | 2/2 
gE |82e laze | SP lee [ale sisae len8 2 aa Pel 
Alga.digana) = Sh ae | ° lgensigses) Eh Reyes ae 
Blackulscaa § S e | 2& |Soaascaal & 6 By ass 
<|O ID = RS fe) a lO iS | & fe o) Pe 
| | | | ae a iz | se 
1 | 8 0.034 0.010 0.024 0.42 8 0.068) 0.014) 0.054 0.26 
a 14 264 0.044) 0.016 0.028, 0.57 14 422 0.078) 0.024| 0.054 0.44 
8 | 12 236 | 0.044) 0.020) 0.024 0.83 16 | 356 | 0.071) 0.024 0.047, 0.51 
UN a) 194 | 0.048 0.021 0.027 0.78 16 324 0.076) 0.026 0.050 0.52 
15, 14 192 | 0.051) 0.023) 0.028) 0.82 16 290 | 0.078) 0.028) 0.050) 0.56 
Le 12, 188 | 0.058) 0.026) 0.032, 0.81 | 16 254 | 0.081) 0.033) 0.048) 0.70 
22)| 12 214 | 0.058) 0.024 0.034 0.71 16 | 214 | 0.078) 0.028 0.050, 0.56 
25 | 12 | 194 | 0.052) 0.024 0.028 0.86 | 14 216 0.075) 0.030 0.045 0.67 
29 14 | 260 0.068 0.032 0.036 0.90 14 174 | 0.084) 0.036) 0.048 0.75 
32 | 14 | 210) 0.062) 0.024 0.038 0.63 | 14 226 | 0.073) 0.024 0.049} 0.49 
36 | 12 206 | 0.068 0.025 0.043) 0.60 12 | 230 | 0.084; 0.024 Ode 0.40 
39 10 | 204 | 0.069) 0.031) 0.088 0.82 8 | 224 | 0.083} 0.030 0.053 0.57 
43 6 212 | 0.081) 0.030) 0.051 0.60, 10 206 | 0.082) 0.032 0.050, 0.64 
* These infusions were prepared in the same manner as the typical members of series A (see below). 
PREPARATION 
OF INFUSIONS® 
Series A: Typical members. Infusions A-1, A-2, A-3, A-4, A-5, A-6, 
A-21, A-22, A-31, A-32, A-41, A-42, A-51° were made essentially 
like those prepared by Peters. 
hay in about 250 ee. of water were boiled five minutes. 
In each case 20 grams Timothy 
Both 
infusion and solid hay were then transferred to a battery jar con- 
taining some unboiled water and the volume made up to 5 
These infusions were than equally seeded with samples 
taken from several cultures of varying ages, in order to insure the 
liters. 
5 For further details see Woodruff, loc cit. 
6 The protozoan count was omitted in A-51. 
