CHEMICAL PROPERTIES OF HAY INFUSIONS Die 
TABLE 5 
Methyl Orange Alkalinity 
(Cubic centimeters of 0.01 N HCl per 100 cc. infusion) 
DAYS 
| | (ee | Fan mieea es 
(ROW te|n2 | 3/4/5/6|7/8/|9 [10 | 11) 12] 13) 14] 15 16 17 18| 19 20} 21| 22} 23| 24| 25| 26) 27| 28 
/ 
+-4|+4|0 +0 +0\—2| 0-4/0 | | 
A-1....;8|9/6/4]6/8)| 8 10) 10) 12) 10) | | | | laeeila'e 
| +i +e+020+2-+9+0-9 40-2 ect eet ae) | 
A-2 | 7} 10) 6) 4) 8) 8 | 10) 12) 12) 14 14) 12) 14) 14) 14 16 | 20 
| : | 
i} 
—2| | | 
RECA 83) Sic8 Wealea| | | | | 16 
| le | Paci 
A-5....| 8 | 10) 6 | | We" | | | 
| +0) —9 | | | | | | 
A-6....| 4 | Kea |pelate ey | | 
| +0 —2\+0 +0 +2 +0 —2/+0 lies | | | 
B-....|7/8|4|6|6|8|6| 10 19 12/12/12 8/19 [12 | | | 12 ete 
+0) | | Wana 
B-2....| 7 | 8 |\4 | | | | Te | 
| +8 +2)+2/+2)+2-42\—2+4 | | pes} | beth | 
C-1....) § | 10] 6 | 6 | 6 | 10] 10) 12| 12) 8 | 12] 12) 8 14 | | 16 | | 22 
| He | | | +4 +2) | | Hes Oi 
C2k 16118 | 10 10 | 10 Keil | | | 
+9)+0—-2+0 | Wwe lah} | 
C-3....| | 10] 6 10 10 10 10 | la at | | | | | 
do not differ materially from the ‘typical members’ of their 
respective groups. From the average of series C in addition to 
the ‘atypical member’ S-1, certain ‘typical members’ C-1, C-3 
and M-1 were also omitted—C-1 because on the fifth day it was 
slightly stirred; C-8 because during the early part of its history 
the hay was at the top; and M-1 because it was stirred at certain 
intervals for a definite purpose, as explained in another place. 
In boiled infusions (series A and B) we can corroborate Peters’!° 
results in regard to the ‘phenolphthalein acidity,’ i.e., a rapid 
rise in which a maximum is reached in from two to six days, 
followed by a more gradual decline; the lowest point being reached 
fifteen to twenty days earlier in series B than in series A (fig. 1). 
The curve for series C is somewhat different; a maximum being 
attained much more gradually. Further reference will be made 
to this below. 
10 Peters: Amer. Journ. Physiol., vol 18, p. 330, 1907. 
