276 MORRIS S. FINE 
Fig. 2 Infusion A-2. illustrating maximum difference in cultures of series A 
between ‘phenolphthalein acidity’ of top and bottom of infusion. Ordinates 
represent number of cubic centimeters of 0.01 N NaOH per 100 ce. of infusion. 
Top = ————_;; bottom = ----- : 
Fig. 3 Infusion C-2, illustrating minimum difference in cultures of series C 
between ‘phenolphthalein acidity’ of top and ‘bottom of infusion. Ordinates 
represent number of cubic centimeters of 0.01 N NaOH per 100 cc. of infusion. 
Top = —————; bottom = - - -- - ; 
at the bottom is hay, whose constituents are dissolving con- 
tinuously, differences of this nature are manifest. However, in 
the course of preparation, a considerable portion of material has 
already entered solution, equilibrium is thus quickly reached and 
acidity differences—never very great—soon disappear. The infu- 
