PLATE 2 
EXPLANATION OF FIGURES 
5 Sperm heads which have been bent so that they are either within or in con- 
tact with the vesicle throughout their entire length. In 6 the flagellum and 
middle piece of the tail are still visible, but in a and ¢ these can no longer be 
made out. Sperm in™ Ringer solution + 0.35 per cent “ NaHCO; for five hours. 
e750! 
6 Sperm heads contained in vesicles; a still showing remains of tail; } 
shows maximum development that the vesicles attain in Ringer solution. Sperm 
in “, Ringer solution four hours. Dark background due to coagulated egg albu- 
men which was mixed with the Ringer solution immediately before fixing te pre- 
vent the vesicles being washed off the cover glass Herla’s. X 1620. 
7 Early stages in the solution and transformation of the sperm heads 
within the vesicle. From same preparation as figs. 1, 2 and 3. 
8 Vesicles in which part of the chromatin is present as discrete granules, 
and part can still he recognized as the portion of the sperm head which adjoins the 
tail. In b two masses of cytoplasm can be seen anteriorly. Herla’s. > 1620. 
9 Sperm heads transformed into nuclei. Chromatin as far as could be 
determined all next to the membrane. Sperm in egg albumen twenty-three hours. 
Gentian violet and balsam. X 5000. 
10 Spermatozoa transformed into nuclei. a@ also appears to have some 
vacuolated protoplasm Sperm in egg albumen twenty-six hours. Herla’s. 
x 3100. 
11 Spermatozoa transformed into nuclei. Chromatin all on nuclear mem- 
brane. Gentian violet and balsam. X 3100. 
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