486 T. H. MORGAN 
becoming diffuse. The polar body is cut off from the egg; its 
nucleus contains five chromosomes, and lying near the nucleus 
is the large lagging chromosome divided into two parts. 
In the next figure, fig. 13, the chromosomes, both in the polar 
body and in the egg nucleus, have fused. The preservation may 
have been poor. The lagging chromosome lies in a vesicle of 
its own to one side of the polar body nucleus. 
The polar body of another egg, fig. 14, shows five chromo- 
somes in its nucleus, three of which at least are elongated as 
though dividing. The lagging chromosome lies outside. Its two 
halves are separated and each half is slightly dumb-bell shaped. 
Three other polar bodies are shown in figs. 15, 16, and 17. 
Each shows the lagging chromosome outside of the nucleus; and 
in two cases surrounded by a partial vacuole of its own. 
In addition to these new cases I have studied and redrawn 
some of the figures given in my former paper, restaining when neces- 
sary to better bring out the chromosomes. One of these, fig. 18, 
shows the equatorial plate of a male egg with one large chro- 
mosome (partly constricted), four intermediate, and one small 
chromosome. ‘Two eases, figs. 19, 20, show polar bodies and 
their contained nucleus and the lagging chromosome outside. 
The third figure, fig. 21, shows the anaphase of an egg that I 
now interpret as a male egg. The interpretation of this anaphase 
figure is difficult because of the presence of a stained body near 
the center of the spindle. After staining and restaining several 
times it seems to me probable that this body is in reality a chro- 
mosome and not a yolk sphere as I formerly thought probable. 
Another sphere lies beyond the outer chromosome group and 
near it another body (in outline in the figure). Both of these 
seem to be in yolk spheres. At the inner pole five distinct chro- 
mosomes are present. If the stained body in the center of the 
spindle be interpreted as a chromosome the spindle bears a close 
resemblance to the spindle of the male-producing egg, but the 
egg is large, and mainly for this reason I was formerly inclined 
to think it a female egg. The case is doubtful and can not be 
interpreted with certainty. 
