* 
554 JACQUES LOEB AND HARDOLPH WASTENEYS 
temperature of 31° differed from that of the fish kept at from 10° 
to 14° (cold room). The fish put in the ice chest had previously 
been at a temperature of 10° for several weeks. 
If we consider the behavior of the fish in an m/8 solution at 31° 
we notice a steady diminution of resistance among those kept at 
0°.4; while among those kept at 10° to 14° the resistance increased 
somewhat. Thelatter resultisnotaccidental. Wemustremember 
that the fish were taken in January when the temperature of the 
water was not far from 0°C. The long exposure to a temperature . 
of from 10° to 14° had therefore a slight immunizing effect. 
Our next task was to ascertain whether fish immunized to resist 
a sudden transfer to a temperature of 35° kept this immunity if 
put on ice just as well as if kept at a temperature of 10° for the 
same period. Our experiments thus far cover only an exposure 
of fourteen days on ice (T. = 0°.4). During this time the immu- 
nity was not diminished, as the following example will show. Fish 
were immunized to a sudden transfer to 35°C. by keeping them 
for two days at 27°. They were then put into a thermostat with 
a constant temperature of 0°.4 for fourteen days and put directly 
TABLE 9 
DURATION OF LIFE AT 31° IN 
RINGER 
SOLUTION 
KEPT AT 0.4° C. 
H:O0 m/32 m/16 m/8 
7 days 26’ 104’ | indef. 
19 days 13’ 42’ ht ‘some indef. | 
33 days | 24’ S07 meme co’ | SSnzaan 
41 days Do 34’ 22 | 8’ | 
, DURATION OF LIFE AT 31° IN | 
= ° EE SA = pe RINGER 
KEPT AT 10° C. 5 canniee 
H20 | m/32 | m/16 m/8 | 
Over 7 days 20’ 135’ indef. 
Over 19 days 13” 26’ | 52’ indef. 
Over 33 days | 4p 86’ /some indef. indef. 
Over 41 days | 41’ some indef. some indef. indef. | 
