340 



W. J. CROZIER 



to be immersed in a given volume of diluted or evaporated sea 

 water were first washed (30 to 60 seconds) in a stream of sea 

 water of the corresponding concentration. The volume of solu- 

 tion containing a single isolated cloacal end was in each case 

 300 cc. 



2.5 50 15 lOO 1^5 



Percen/h^e d/Z/y/^/on o/ seacoaterr 

 Fig. 29 Based on experiments with 45 specimens of Stichopus 



Five individual pieces were carefully studied at each concen- 

 tration of sea water and their behavior checked by less detailed 

 observations upon a number of other examples. The average 

 times for the continuation of pulsation and of irritability to 

 mechanical stimulation under the conditions just specified (at 

 25° to 27°) are given graphically in figure 29. As regards the 

 condition of the cloacal sphincter after the cessation of pulsa- 

 tion, this difference was noted between the action of rain water 



