564 ASA A. SCHAEFFER 



out toward the test object. Both the main and the side pseudo- 

 pods rolled the carmine around for three minutes. Then what 

 was previously the side pseudopod became the main one — 169. 

 The ameba then ingested the carmine in a typical food cup but 

 with a very small quantity of water. The food cup was formed 

 by throM'ing out two small pseudopods around the carmine, fol- 

 lowed by a sheet of protoplasm thrown over the carmine. There 

 was no period of rest, the ameba continuing forward in the same 

 direction in which it moved before ingestion. Seven minutes 

 after eating it the carmine was excreted. 



Six minutes later the same grain of carmine was laid near the 

 forward end of the ameba — 176. A pseudopod directed toward it 

 enlarged and finally came into contact with it. A food cup was 

 at once formed and the carmine ingested for the third time with 

 a moderate amount of water. The ameba did not become quiet 

 after eating the carmine, but continued moving forward in the 

 same direction. About seven minutes after eating the carmine 

 it was excreted and left behind. 



A few minutes later the carmine grain was placed near the 

 ameba for the fourth time — 187 — but there was no definite reac- 

 tion toward it. When laid near the ameba for the fifth time a 

 positive reaction was produced, but after coming into contact with 

 the carmine, the ameba resumed its original direction of move- 

 ment. A flagellate then happened to hover near the carmine 

 198 — which induced the prompt formation of a food cup over it 

 and partly over the carmine. The flagellate was taken into the 

 protoplasm but the carmine, having been at no instant entirely 

 surrounded, was left outside. 



The sixth presentation of the carmine grain produced very 

 slight changes of behavior — 202. Slight but definite reactions 

 were brought about but the ameba finally moved on without 

 change of direction, leaving the carmine behind. The seventh 

 trial produced results similar to those of the sixth — 211. In the 

 eighth trial, with the same carmine grain, only a very slight posi- 

 tive reaction of short duration was produced — 218. 



A piece of carmine of grade 2 was then laid sHghtly to the left 

 of the advancing tip of the ameba — 221. The ameba moved for- 



