428 Max Mapes Ellis 
Experiment 3. Food, Spirogyra and Raw Beef 
Some months after the conclusion of Experiment 2, another set of 
tadpoles were subjected to the same conditions used in Experiment 
2. ‘[wo other series at temperatures not used in Experiment 2 
were added. One of these was maintained at 96° F. and the other 
at 103° F. ‘Table 4 is made up from the data collected in this 
experiment. [he measurements were taken but once, the twelfth 
day after the operation. 
TABLE 4 
TEMPERA- ree AMOUNT PER CENT 
TURE REGENER- | REGENER- 
oF: Ee ATED ATED 
| mm. mm. 
(| 66 2 0.6 30 
SEL eRE De Pan Pese te wharaye fata "s vocrw widin ole we { | 66 5 | os 32 
{ 66 10 3-0 32 
Petes tet a Se An es y=) 45 
Names: |[osqsodutcdas din deeatacseon gamete } 76 5 2.2 44 
( 76 10 4-5 45 
SIEWOS LiGaedcraioes ecco ORCS OO eae en eee 57 | all animals active; no deaths; no regenera- 
tion. 
SSsG8 Ie caso abion tie AocRee Cae oe ROR 47 | all animals active; no deaths; no regenera- 
tion. 
Series! Vicnvaciiere teihelstaa ice ee von eeareels 96 all animals dead in 24 hours after opera- 
| tion. : 
EXIGE Neral etsy a ilye ices cise, os aeeGiatne she | 103 | all animals dead in less than 12 hours 
| | after operation 
It was hoped that tadpoles would live at a temperature higher 
than 76° F. but unfortunately, the two temperatures above 76 
were too high to sustain life. There is the same proportional 
relation between the amount removed and the amount regener- 
ated at the end of the twelfth day, as shown by these data as 
has been previously noted. The per cent regenerated in this 
experiment was lower than that regenerated in Experiment 2, 
which shows a variation at some point between the conditions 
controlling the two experiments. A possible cause of this differ- 
ence may be the influence of the season upon tadpoles’ ability 
