694. A. F. Goldfarb 
segments sloughed off, either at the time of operating or after 
several days. Owing to this autotomy the regeneration of the 
new tail began not from the cordless level but from the more 
anterior regions, where the organs were uninjured. In every case 
examined, the injured, and often many of the uninjured segments 
as well, had been cast off. In No. 3.6 for example the piece con- 
tained 85 segments. The cord had been removed from the pos- 
terior 8 segments. At the time of fixation however there were but 
73 segments. In this animal 8 injured as well as 4 uninjured 
Fig. 14 Nerve cord of the earthworm, extracted according to method described on page 693. 
Drawn from fresh specimen. 
segments had sloughed off. No further work on posterior level 
was carriedon. Autotomy near the anterior endof the worm was 
very rare and every such case was discarded. ‘The 5th segment 
with its reproductive opening offered a very convénient point from 
which the number of segments could be counted. 
The earthworms were isolated, in separated flower pots, con- 
taining damp earth. They were placed on top of the earth and 
remained there for 25 to 40 days. When stimulated they reacted 
sluggishly. Beginning about 25 days after the operation, some 
Ee ™ = oe 
ee 
