EFFECT OF CONJUGATION 285 



the moist chambers, the latter are examined and the records on 

 the sHdes copied to permanent records on large sheets of paper. 

 From these records the exact number of fissions can be obtained 

 at any time; thus, since in the above case the generation contain- 

 ing 16 had been obtained from 2, it is clear that three fissions 

 had occurred. 



Tabulation and tables. For analysis, the records of. fissions 

 have in most cases been tabulated for definite periods, as of one 

 week or of ten days. In analyzing the records it has been nec- 

 essary to make from the original tables of records a very large 

 number of secondary tables, particularly correlation tables. In 

 place of publishing these secondary tables, the original tables of 

 record will be pubhshed in the Appendix of the present paper. 

 These contain all that would be found in the correlation and 

 other secondary tables, and anyone who desires can reconstruct 

 the latter from them, so that they furnish every possibility for 

 testing the results here given. Furthermore, the original record 

 tables show much that is lost when they are transformed into 

 correlation tables; particularly do they show much that is of 

 interest from the point of view of 'pure line' studies. 



Besides these tables giving the original records, the present 

 paper will contain as a rule only tables giving the data — the 

 constants, et cetera — resulting from the analysis, by biometrical 

 methods, of these records; these are found in the body of the paper. 



III. EFFECT OF CONJUGATION ON MULTIPLICATION, SURVIVAL AND 



VARIATION 



As previously noted, the experiments described below furnish 

 evidence on other matters besides those set forth in the heading 

 above, but we shall examine them first from this point of view. 

 We shall divide the experiments into two sets; the first including 

 those dealing with 'wild' cultures; the second those dealing with 

 pure strains. 



