2 Ada Springer 



The total number of individuals used in this and the subsequent 

 investigation was about three hundred and seventy-five. All 

 of the salamanders were adults, at least two and one-half or three 

 years old, for, according to Gage^ "the autumn of the third 

 or the spring of the fourth year after hatching when two and one- 

 half or three years old, the animal enters the water and assumes 

 an aquatic life." All the animals used in the experiments, with 

 the exception of six sets in Part I used to determine the effects 

 of temperature, were taken from the same environment. 



The animals were kept in sets consisting of six, ten and five 

 individuals, respectively. For convenience the various sets are 

 designated by capital letters, A, B, C; and when one set is a 

 duplicate of another this fact is shown by designating those sets 

 by capitals with the addition of the numerals, A^ A^ etc. The 

 animals were kept in flat dishes containing from one and one-half 

 to two inches of water, the room temperature varying from 20° 

 C. to 25° C. 



Pieces of raw beef of a definite size were used for food. 

 Although an attempt was made to keep the pieces uniform, the 

 size varied somewhat; that is, both the size of the pieces given 

 to different animals at the same feeding and also the size of the 

 pieces given to one animal at different feedings. A comparison 

 of records, taken at random, of the amount of beef fed to the 

 individuals at the same and at different feeding periods (the 

 amount fed to the individual being determined by taking the 

 average of the weight of beef given to the set), shows the follow- 

 ing variations. The weight is expressed in milligrams. 



Three feeding periods: 



(i) 44—58 — 47 — -52 — 55; average for the period, 51 mg. 



(2) 61 — 52 — 61 — 59 — 63 — 56 — 68 — 65 — 53 — 60; average, 59 mg. 



(3) 49—49—45—55' average, 49 mg. 



During the first, second and possibly the third week after the 

 beginning of the experiments the average amount of beef fed to 

 the individuals was lower than the averages given above, because 



* The life of the vermilion-spotted newt (Diemyctylus viridescens). 



