lo8 N. M. Stevens 



The two smallest are the heterochromosomes. The synizesis 

 and synapsis stages are of the loop type, though not especially 

 clear. Fig. 74 is the equatorial plate of the first maturation divi- 

 sion; and Figs. 75 to yy, metaphase and anaphase, show the une- 

 equal pair of heterochromosomes dividing either earlier or later 

 than the other chromosomes. 



Only one specimen of Cylene gave any maturation divisions. 

 The remainder of the testes examined contained only spermatids 

 and spermatozoa. The number of chromosomes is the same as in 

 Tetraopes, twenty. The larger heterochromosome shows the 

 peculiarity of holding the stain longer than the other chromosomes. 

 Figs. 78 and 79 are metaphases of the first division from an iron 

 haematoxylin preparation much destained. 



EPICAUTA CINEREA (fAM. MELOID^) 

 EPICAUTA PENNSYLVANICA (fAM. MELOID^) 



Two varieties of Epicauta cinerea, one with all gray elytra and 

 the other with a lighter gray border around the elytra, were 

 studied, and the chromosomes in both, as well as in Epicauta 

 pennsylvanica, found to be of the same number and character — 

 nineteen large and one small chromosome in the spermatogonia. 

 The synizesis and synapsis stages are of the loop type and the 

 maturation divisions result in spermatids one half of which con- 

 tain the small heterochromosome, and one half the large one. 

 Figs. 80 to 83 show the chromosomes of the spermatogonia, first 

 and second spermatocytes. The larger heterochromosome holds 

 the stain as in Cylene. 



PENTHE OBLIQUATA (fAM. MELANDRYID^) 



Only one pair of Penthe obliquata has been captured. The 

 ovaries and one testis were fixed in Gilson's mercuro-nitric fluid, 

 and the other testis in Flemming. The Flemming material 

 alone gave any satisfactory results. The presence of an unequal 

 pair of heterochromosomes is shown in Figs. 84 to 86 — a sperma- 

 togonial plate, a section of a first spermatocyte spindle, and two 

 second spermatocyte equatorial plates. 



