Maturation of Parthenogenetic and Sexual Eggs 1 43 



C-D), could they be counted because of being too closely crowded 

 together. The polar view of the metaphase in the alcohol acetic 

 fixation gave the best results. Fourteen chromosomes were seen 

 in several sections of different eggs (Fig. 5, A-B). The chromo- 

 somes were of about the same size as those in the metaphase of 

 the male parthenogenetic egg and were much larger in size and 

 less in number than those in the metaphase of the female partheno- 

 genetic egg. 



\ p 



Fig. 5 Winter or fertilized egg. A, B, equatorial plates of the polar spindle, showing fourteen 

 chromosomes; C, D, anaphases of the polar spindle, showing twelve to fourteen chromosomes on each 

 end of the spindle. 



On account of the thick and opaque egg envelope, the polar 

 bodies were never seen. 



VI GENERAL DISCUSSION 



Although Lenssen was mistaken in regard to the number of the 

 chromosomes nevertheless he was firmly convinced that the num- 

 ber in the maturation stages of the male parthenogenetic and 

 the fertilized egg were the same and that the number in the female 

 parthenogenetic egg was greater. By comparing my Figs, i, 3 

 and 5, it will be seen that this conclusion is confirmed. The 

 greatest number of chromosomes seen in an equatorial plate of 

 the male egg was possibly thirteen and the number seen in an 

 equatorial plate of a winter egg was fourteen. The chromosomes 

 of both eggs in the same stages were of the same size. 



In the female parthenogenetic egg the greatest number of chro- 

 mosomes seen was twenty-five (Fig. i). The chromosomes were 



