The Life History of Tillitia Magna 405 



times of depression. Accordingly, six sets of experiments were 

 started. 



Experiment i. B5, B6, were treated with sugar. 



Experiment 2. B7 and B8 were treated with tap water. 



Experiment ^. B9, Big, Bii, Bi2, were treated with KjHPO^. 



Experiment ^. B13, B14, B15, B16, were treated with pan- 

 creatin. 



Experiment ^. B17, B18, B19, B20, were treated with beef 

 extract. 



Experiment 6. B25, B26, B27, B28, were treated with alcohol. 



The records for the sugar experiments are as follows : February 

 26, B5 and B6, from stock of Bi and B2, were put into a solution 

 of y\ sugar plus tap water; February 27, individuals were pale, 

 but normal; fresh solution was given; February 28, both individ- 

 uals encysted as if for division; March 2, B5 divided unequally, 

 and B6 encysted permanently; March 5, both lost. 



Thinking there might be a sufficient amount of salts in ordi- 

 nary tap water to sustain life, and disregarding the difference in 

 densit}- also, since many well-fed individuals that had become 

 sluggish, had resumed their normal activity when put in tap 

 water, a water culture of two lines, B7 and B8, was started from 

 the stock of B3 and B4. These are the records: 



February 26. By and B8 transferred to tap water. 



February 27. By and B8 divided once; individuals pale, and were put in fresh water. 



February 28. By encysted for division; B8 divided twice. 



March i. By divided three times, B8 divided once. 



March 2. No division. 



March 3. No division; both permanently encysted. 



In the case of the experiments with potassium phosphate, four 

 lines were started from the stock of Bi, B2, B3, B4. Each was 

 put in a solution containing ten drops of medium and one drop 

 of J^ /_ sol. of H2PO4. B9 was left in the solution one day, 

 Bio two days, Bii three, and B12 four days. B9 and Bio were 

 lost, and the lines were filled in from B12. At the end of the 17th 

 period, a second treatment was given, but this had no effect, and 

 the lines died out at the end of the 20th period. 



