524 



R. W. Hesner 



fixation. The sections also show a similar arrangement of mate- 

 rials. Fig. 14 represents a transverse section through C.B. 10, c. 

 There is a light vesicular zone at the side which was turned toward 

 the axis of rotation; this is folded into bud-like prominences just 

 as we found to be the case in C.B. 4, e, and others. The yolk- 

 globules are distributed in the usual manner, the large ones being 

 on the heavy side. The "Keimhautblastem" has moved away 

 from the side of greater specific gravity and toward the hghter 

 side. No gray cap could be found. It is probable that the 

 material which produces this zone has all been thrown to the outer 

 side, but the area is too great to allow of any perceptible accumula- 

 tion. 



C.B. 10, e, f, g and h. No one of these eggs developed beyond 

 an early cleavage stage. The nuclei then disintegrated and the 

 amoeboid masses of cytoplasm in which they lay became vacuo- 

 lated as did also the "Keimhautblastem." 



TABLE V 



CaUigrapha higshyana — Series C.B. 9 



Series C.B. 9 — Table V 



Although these eggs were taken as soon as laid, sections through 

 C.B. 9, a show that they were in a rather advanced cleavage stage 

 when the experiments were begun. They represent a condition 

 intermediate between those of series C.B. 2 (Fig. 17) and C.B. 4 

 (Fig. 9), resembling in structure an egg ten hours old. 



