580 y. Frank Daniel 



I wish here to express my sincerest appreciation to Professor 

 Morse, of the Johns Hopkins Chemical Laboratory, for his help- 

 fulness in planning a constant temperature apparatus highly satis- 

 factory for this purpose. 



B Experimental 



With the various sources of error controlled in the way just set 

 forth, the two types E and F may now be studied in detail. 



I Study of Type E 



In my attempts at acclimatizing Stentors of type E to alcohol 

 various plans have been resorted to. These will be described in 

 their proper connections. 



Both methods by which type F was readily immunized to ethyl 

 alcohol were tried for type E. The method of testing the ability 

 of alcoholized animals to live in a stronger percentage of alcohol 

 gave slight results for type E. Although this type was trans- 

 ferred with great care it was dijB&cult to get it to live in a stronger 

 medium than 1.5 per cent in strength. The other method in 

 which the resistance period of the normal and acclimatized animals 

 was compared, was adopted as the more satisfactory of the two. 

 To test for immunity by this latter method the animals of type E 

 were allowed to remain for short periods of time in a I per cent 

 solution made by adding 6 per cent ethyl alcohol to their normal 

 culture medium.^'' The organisms were then tested as to their 

 resistance to a fatal dose. 



The results of the tests are shown in the experiments which 

 follow : 



'^Different percentages were tried for making up the acclimatizing medium and 6 per cent was adopted 

 as less likely to produce injury when first added. 



