584 



J. Frank Daniel 



Experiment VII 



RESISTANCE OF STENTORS OF TYPE E TO 8 PER CENT ALCOHOL, AFTER LIVING 4 DAYS IN 1. 5 PER CENT 



ALCOHOL 



Exp. I c 



Z CI 



3 



5ci 

 6 



7 c: 



8 ci 



9 ci 

 10 c 



A Four Days in 7.5 Per Cent. 



Seconds 



lia stop 40 



lia stop 90 



lia stop 45 



lia Stop 75 



lia stop 30 



lia stop 35 



lia stop 45 



lia stop 35 



lia stop 50 



lia stop 35 



Average resistance = 48 . 



C Control 



Seconds 



Exp. I cilia stop 50 



2 cilia stop 35 



3 cilia stop 75 



4 cilia stop 55 



5 cilia stop 30 



6 cilia stop 105 



7 cilia stop 130 



8 cilia stop 115 



9 cilia stop 35 



10 cilia stop 25 



Average resistance = 65.5 



In Experiment VII, although the animals had been kept in the 

 strongest medium that they could withstand, still no immunity 

 was shown. On the contrary, the alcoholized animals were actu- 

 ally less resistant than were those of the control. 



Briefly summarizing the results of the five experiments (III to 

 VII) it is to be noted that only III and IV gave evidence of 

 immunity; that V, although positive in resistance, was so 

 low as to fall easily within the experimental error; while VI 

 showed no increase whatsoever, and VII gave a higher resist- 

 ance in its control than in its alcoholized animals. 



It will be observed that the foregoing series of experiments on 

 'type E were made from the third to the fifth days of acclimatiza- 

 tion. The same general results are obtained, however, if tested 

 either earlier or later. 



A final study of this type may be added in which tests were made 

 after the second, fifth and seventh days of acclimatization. These 

 animals were reared in a fluid gradually brought up to i per cent, 

 and were tested to a 6 per cent solution of alcohol. 



The results are briefly shown in seconds of resistance in the 

 following study made on the diff"erent days : 



