594 J- Fray^k DajTiel 



This behavior may be shown in no better way than by a detailed 

 description of what took place in a single individual. This cell 

 is given in preliminary Experiment i (individual 4), and is 

 entirely typical of the general proceedings, excepting that its 

 resistance period was unusually long. 



Upon first subjection to the killing fluid the body of this cell 

 remained motionless; at the end of 10 seconds a strong antero- 

 lateral bulging occurred in the aboral region; the cilia continued 

 to beat up to 45 seconds though their action was slow and uncer- 

 tain. After surviving the shock incident to subjection to the fluid, 

 a gradual increase in strength of stroke of the cilia followed, which 

 finally caused the whole mass of protoplasm to vibrate in wave- 

 like rhythm, until within a few seconds of death (at 720 seconds). 



We may then say that the effects of strong alcohol upon acclim- 

 atized animals of type F were different from those upon type E 

 in at least three ways : (i) In showing a higher degree of increase, 

 (2) in producing greater constancy of action, and (3) in manifest- 

 ing a definite method of reaction in the protoplasm. 



With these considerations we may now pass to a study of another 

 cell with the single remark that never during the months that I 

 have worked upon type E, have I been entirely able to assure 

 myself of its adjustment to alcohol, and at no time have I had 

 reason to doubt such adjustment in type F. 



4 A Study of Spirostomum 



A further study of acclimatization to alcohol was made upon one 

 of the largest of the single celled organisms, Spirostomum (S. 

 ambiguum). 



While this form is not found attached as in the case of Stentor, 

 the disadvantage due to its activity is fully compensated for by 

 the most definite of death-points. Subjected to a chemical stim- 

 ulus Spirostomum begins backing, as is characteristic of many of 

 the ciliates. If the chemical into which the organism is introduced 

 be of sufficient strength to cause death the first sign of injury ap- 

 pears as a rupture at the extreme anterior end. Disintegration, 

 beginning at this point, travels rapidly towards the posterior end 



