Immunity of Lower Organisms to Ethyl Alcohol 597 



From experiments in an 8 per cent solution, another point of 

 extreme interest was observed. 



Organisms often showed the first signs of injury within the 

 usual time — one to two minutes — and disintegration followed in 

 the usual way. Upon reaching mid-body, however, this was 

 suddenly stopped. At the point of injury a round plug of proto- 

 plasm formed, filling up the wound. Thereupon the cilia resumed 

 a backward stroke and the body moved forward in a normal 

 fashion. 



This phenomenon was observed again and again as the method 

 by which the organisms often prolonged life for considerable 

 periods of time. 



In the same way that type E of Stentor was tested on the second, 

 fifth and seventh days, we may test Spirostomum to see the general 

 indications of resistance. 



These results, shown in a condensed way in the following table, 

 are different from those obtained in type E of Stentor. 



Experiment XIII 



RESISTANCE OF SPIROSTOMA TO 8 PER CENT ALCOHOL AFTER LIVING IN I PER CENT ALCOH OL. A = LIVING 

 IN I PER CENT ALCOHOL. C = CONTROL 



Second Day Fifth Day Seventh Day 



In the foregoing series only a slight increase for the acclimatized 

 animals is shown on the second day. It will be noted, however, 

 that the normal resistance (104 seconds) is high. In other cases 



