6o8 J. Frank Daniel 



due to acclimatization in i per cent alcohol. The resistance to - 

 glycerin was decreased by remaining in alcohol. 



In a weaker solution, however, while Spirostomum showed a 

 similar specificity, Stentor was far less regular. In an | solution 

 in one case it showed a slight advantage in its acclimatized ani- 

 mals (A = 516'', C =- 462'') • Study in a weaker solution (m) gave 

 results still more difficult to interpret. Animals reared in i per 

 cent alcohol gave a resistance to f glycerin of 1293 seconds; 

 those in 0.5 per cent, 1696 seconds, and those reared in normal 

 culture medium, 1600 seconds. 



Even in these cases a certain degree of specificity of immunity 

 is evident, for in no place is there a degree of increase in the resist- 

 ance to glycerin approximating that given to ethyl alcohol. 



E The Action of Methyl Alcohol {CHfiH) 

 I General Effects 



In methyl alcohol, a lower member of the alcohol group, the 

 following condition was found: 



Normal unacclimatized Stentors subjected to an 8 per cent solu- 

 tion: Movement slight or animal altogether quiet; body bulging 

 antero-Iaterally much as in the case of ethyl alcohol. Loss of 

 body color; strongly marked distortion in 80 to 105 seconds; 

 membranellae around groove persistent; frontal field retaining 

 color and its cilia active after the posterior part of the body was 

 colorless. 



Acclimatized Stei^tors. Stentors acclimatized in i per cent 

 ethyl alcohol when subjected to 8 per cent methyl alcohol showed 

 the following effects : 



Early movement; membranellae stopped somewhat as in 

 glycerin; an occasional animal with membranellae lost, but the 

 peristome was not cast off in a ribbon-like band as occurred in 

 glycerin. 



Slight, if any, difference could be seen in the behavior of the 

 acclimatized and the control animals in the above comparison. 

 In both, the membranellae had a resistance mid-way between that 

 shown in ethyl alcohol and in glycerin. 



